georefid:2007-008191georefid:2007-008188SEDIS Publication Catalogue

ana.macario@awi.de

http://sedis.iodp.org/pub-catalogue/informationpointOfContact2011-06-23T00:00:00Zhttp://sedis.iodp.org/pub-catalogue/index.php?id=10.2973/odp.proc.sr.201.107.20052006-01-01publicationgeorefid:2007-008191doi:10.2973/odp.proc.sr.201.107.2005Biddle, Jennifer F.Pennsylvania State University, Department of Geosciences, University Park, PA, United StatesauthorHouse, Christopher H.University of Rhode Island, United StatesauthorBrenchley, Jean E.Ocean Drilling Program, United StatesauthorTexas A&M University, Ocean Drilling Program, College Station, TX, United StatespublisherdocumentDigitalIn: Jorgensen, Bo B. (editor), D'Hondt, Steven L. (editor), Miller, D. Jay (editor), Aiello, Ivano W., Bekins, Barbara, Blake, Ruth E., Cragg, Barry A., Cypionka, Heribert, Dickens, Gerald R., Ferdelman, Timothy G., Ford, Kathryn H., Gettemy, Glen L., Guerin, Gilles, Hinrichs, Kai-Uwe, Holm, Nils G., House, Christopher H., Inagaki, Fumio, Meister, Patrick, Mitterer, Richard M., Naehr, Thomas H., Niitsuma, Sachiko, Parkes, R. John, Schippers, Axel, Skilbeck, C. Gregory, Smith, David C., Spivack, Arthur J., Teske, Andreas P., Wiegel, Juergen, Proceedings of the Ocean Drilling Program; scientific results; controls on microbial communities in deeply buried sediments, eastern Equatorial Pacific and Peru Margin; covering Leg 201 of the cruises of the drilling vessel JOIDES Resolution; San Diego, California, to Valparaiso, Chile; Sites 1225-1231; 27 January-29 March 2002201The deep biosphere is estimated to hold a significant percentage of the Earth's prokaryotic biomass; however, little is known about the organisms in this environment. Here, we describe investigations of the diversity of microorganisms enriched from surface and subsurface sediment collected during Leg 201 of the Ocean Drilling Program at Site 1230 on the slope of the Peru Trench. This site contains methane hydrates, high levels of organic matter, and high direct cell counts, all of which indicate the potential for thriving microbial populations. To investigate these populations, we examined prokaryotes in samples from seafloor to 258 meters below seafloor (mbsf) using both cultivation and molecular methods. From seafloor samples, we cultivated isolates representing the genera Photobacterium, Shewanella, and Halomonas. The population found in an enrichment cultivated at low temperatures, 0.67 mbsf, contained many cell morphologies and deoxyribonucleic acid (DNA) signatures, but this population, except for a Vibrio sp., was difficult to separate and grow as pure cultures. Most isolates produced extracellular lytic enzymes that were active at low temperatures. Methanogens have been expected to play a large role in the creation of methane hydrates in the sediment; therefore, we also attempted to enrich for psychrophilic methanogens. No methane was found above background levels in anaerobic enrichments incubated for 2 yr, nor was any 16S ribosomal DNA detected following amplification using archaeal primers with DNA extracted from these incubated cultures. These results illustrate the need for further extensive microbiological studies in order to understand the biogeochemistry of this important subseafloor environment.completedOceanographybiochemistrybiomasscontinental margincontinental slopecoresdeep-sea environmentDNAEast PacificecologyEquatorial Pacificexperimental studiesgeochemistryLeg 201living taxamarine environmentmarine sedimentsmicroorganismsOcean Drilling ProgramODP Site 1230Pacific OceanPeruPeru-Chile TrenchphylogenyprokaryotessedimentsSouth Americaurn:org.iodp:exp:201urnlargerWorkCitationcampaignurn:org.iodp:exp:201:site:1230urnlargerWorkCitationstudyurn:org.iodp:exp:201urnlargerWorkCitationcampaignEnglishgeoscientificInformation-80.3500-80.3500-9.0700-9.0700Available only on CD-ROM in PDF format and on the Web in PDF or HTMLapplication/pdfunknownSEDIS Publication Catalogue

ana.macario@awi.de

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