Upper Eocene impact ejecta has been discovered all over the world. The number of upper Eocene impact layers and the geographic distribution of each layer, based on major chemical composition and biostratigraphic data, are not agreed upon. We have performed four Sr-Nd isotopic analyses of clinopyroxene-bearing spherules (cpx spherules) and three Sr-Nd analyses of microtektites from five Deep Sea Drilling Project/Ocean Drilling Program (DSDP/ODP) sites in the South Atlantic and Indian Oceans. Our data support the hypothesis that there is only one cpx spherule layer in upper Eocene sediments. We also find that the microtektites associated with the cpx spherule layer in the South Atlantic and Indian Oceans are not part of the North American tektite strewn field, but belong to the same event that produced the cpx spherules. The microtektites, together with cpx spherules, are more heterogeneous than microtektites/tektites from other strewn fields. No direct link has been established between the microtektites from this study and possible target rock at the Popigai crater.