The Chesapeake Bay impact structure, which is about 35 Ma old, has previously been proposed as the possible source crater of the North American tektites (NAT). Here we report major and trace element data as well as the first Sr-Nd isotope data for drill core and outcrop samples of target lithologies, crater fill breccias, and post-impact sediments of the Chesapeake Bay impact structure. The unconsolidated sediments, Cretaceous to middle Eocene in age, have epsilon (sub Sr) (super t = 35.7 Ma) of +54 to +272, and epsilon (sub Nd) (super t = 35.7 Ma) ranging from -6.5 to -10.8; one sample from the granitic basement with a T (super Nd) (sub CHUR) model age of 1.36 Ga yielded an epsilon (sub Sr) (super t = 35.7 Ma) of +188 and an epsilon (sub Nd) (super t = 35.7 Ma) of -5.7. The Exmore breccia (crater fill) can be explained as a mix of the measured target sediments and the granite, plus an as-yet undetermined component. The post-impact sediments of the Chickahominy formation have slightly higher T (super Nd) (sub CHUR) model ages of about 1.55 Ga, indicating a contribution of some older materials. Newly analyzed bediasites have the following isotope parameters: +104 to +119 (epsilon (sub Sr) (super t = 35.7 Ma) ), -5.7 (epsilon (sub Nd) (super t = 35.7 Ma) ), 0.47 Ga (T (super Sr) (sub UR) ), and 1.15 Ga (T (super Nd) (sub CHUR) ), which is in excellent agreement with previously published data for samples of the NAT strewn field. Target rocks with highly radiogenic Sr isotopic composition, as required for explaining the isotopic characteristics of Deep Sea Drilling Project (DSDP) site 612 tektites, were not among the analyzed sample suite. Based on the new isotope data, we exclude any relation between the NA tektites and the Popigai impact crater, although they have identical ages within 2sigma errors. The Chesapeake Bay structure, however, is now clearly constrained as the source crater for the North American tektites, although the present data set obviously does not include all target lithologies that have contributed to the composition of the tektites.