Morono, Yuki et al. (2011): Preliminary experiment for cell count using flow cytometry

Leg/Site/Hole: Related Expeditions:

IODP 329
IODP 329 U1365
IODP 329 U1366
IODP 329 U1367
IODP 329 U1368
IODP 329 U1369
IODP 329 U1370
IODP 329 U1371

Identifier: ID:

2012-014432

Type:

georefid

ID:

10.2204/iodp.proc.329.110.2011

Type:

doi

Creator: Name:

Morono, Yuki

Affiliation:

Japan Agency for Marine-Earth Science and Technology, Kochi Institute for Core Sample Research, Kochi, Japan

Role:

author

Name:

Kallmeyer, Jens

Affiliation:

Japan Agency for Marine-Earth Science and Technology, Japan

Role:

author

Name:

Inagaki, Fumio

Affiliation:

Integrated Ocean Drilling Program, United States

Role:

author

Name:

D'Hondt, Steven

Affiliation:

Lamont-Doherty Earth Observatory, United States

Role:

author

Name:

Alvarez Zarikian, Carlos

Affiliation:

Dalhousie University, Canada

Role:

author

Name:

Evans, Helen

Affiliation:

Carl von Ossietzky Universitaet Oldenburg, Germany

Role:

author

Name:

Dubois, Nathalie

Affiliation:

Max-Planck-Institute of Marine Microbiology, Germany

Role:

author

Name:

Engelhardt, Tim

Affiliation:

Aarhus Universitet, Denmark

Role:

author

Name:

Ferdelman, Timothy

Affiliation:

Oregon State University, United States

Role:

author

Name:

Gribsholt, Britta

Affiliation:

Minnesota State University-Mankato, United States

Role:

author

Name:

Harris, Robert N.

Affiliation:

Hanyang University, South Korea

Role:

author

Name:

Hoppie, Bryce W.

Affiliation:

University of Potsdam, Germany

Role:

author

Name:

Hyun, Jung-Ho

Affiliation:

Yonsei University, South Korea

Role:

author

Name:

Kim, Jinwook

Affiliation:

University of Melbourne, Australia

Role:

author

Name:

Lynch, Jill E.

Affiliation:

Shizuoka University, Japan

Role:

author

Name:

Mitsunobu, Satoshi

Affiliation:

Boston University, United States

Role:

author

Name:

Murray, Richard W.

Affiliation:

University of Tsukuba, Japan

Role:

author

Name:

Reese, Brandi K.

Affiliation:

Kyushu University, Japan

Role:

author

Name:

Shimono, Takaya

Affiliation:

Hiroshima University, Japan

Role:

author

Name:

Shiraishi, Fumito

Affiliation:

University of Southampton, United Kingdom

Role:

author

Name:

Smith, David C.

Affiliation:

University of Bergen, Norway

Role:

author

Name:

Smith-Duque, Christopher E.

Affiliation:

AIST/Geological Survey of Japan, Japan

Role:

author

Name:

Spivack, Arthur J.

Affiliation:

Dublin City University, Ireland

Role:

author

Name:

Steinsbu, Bjorn Olav

Affiliation:

IFREMER, France

Role:

author

Name:

Suzuki, Yohey

Affiliation:

Chiba University, Japan

Role:

author

Name:

Szpak, Michal

Affiliation:

University of Tokyo, Japan

Role:

author

Name:

Toffin, Laurent

Affiliation:

Chinese Academy of Sciencesa, South China Sea Institute of Oceanology, China

Role:

author

Name:

Uramoto, Goichiro

Affiliation:

Ocean University of China, China

Role:

author

Name:

Yamaguchi, Yasuhiko

Affiliation:

University of Southern California, United States

Role:

author

Name:

Zhang Guoliang

Affiliation:

University of Potsdam, Germany

Role:

author

Name:

Zhang Xiaohua

Affiliation:

University of Rhode Island, United States

Role:

author

Name:

Ziebis, Wiebke

Affiliation:

Integrated Ocean Drilling Program, United States

Role:

author

Identification: Title:

Preliminary experiment for cell count using flow cytometry

Year:

2011

Source:

In: D'Hondt, Steven, Inagaki, Fumio, Alvarez Zarikian, Carlos, Evans, Helen, Dubois, Nathalie, Engelhardt, Tim, Ferdelman, Timothy, Gribsholt, Britta, Harris, Robert N., Hoppie, Bryce W., Hyun, Jung-Ho, Kallmeyer, Jens, Kim, Jinwook, Lynch, Jill E., Mitsunobu, Satoshi, Morono, Yuki, Murray, Richard W., Reese, Brandi K., Shimono, Takaya, Shiraishi, Fumito, Smith, David C., Smith-Duque, Christopher E., Spivack, Arthur J., Steinsbu, Bjorn Olav, Suzuki, Yohey, Szpak, Michal, Toffin, Laurent, Uramoto, Goichiro, Yamaguchi, Yasuhiko, Zhang Guoliang, Zhang Xiaohua, Ziebis, Wiebke, Proceedings of the Integrated Ocean Drilling Program; South Pacific gyre subseafloor; Expedition 329 of the riserless drilling platform; Papeete, Tahiti, to Auckland, New Zealand Sites U1365-U1371, 9 October-13 December 2010

Publisher:

IODP Management International, Washington, DC, United States

Volume:

332

Issue:

Pages:

Abstract:

One of the major challenges in microbial ecology is to evaluate the accurate number of living cells in a natural environment. Cell count using flow cytometry is a powerful, high-throughput technique that has widely been used for aquatic habitats but not for sedimentary environments because mineral grains interfere with cell detection. During Integrated Ocean Drilling Program Expedition 329, we tested several sample preparation methods for onboard cell counting using flow cytometry with various pelagic sediments from the South Pacific Gyre. The cell numbers acquired from shallow sediments are almost consistent with microscopic direct counts (i.e., approximately 105 cells/cm (super 3) ). Yet, the method needs improvement and standardization to reduce the background signal and to lower the minimum detection limit for deep sedimentary habitats with very low cell densities (i.e., <1000 cells/cm (super 3) ).

Language:

English

Genre:

Serial

Rights:

URL:

http://publications.iodp.org/proceedings/329/EXP_REPT/CHAPTERS/329_110.PDF

Coverage:Geographic coordinates:

	North:-23.5100
West:-165.3900		East: -123.0900
	South:-45.5800

Keywords:

Oceanography; boreholes; cores; detection; ecology; Expedition 329; experimental studies; flow cytometry; geomicrobiology; Integrated Ocean Drilling Program; IODP Site U1365; IODP Site U1366; IODP Site U1367; IODP Site U1368; IODP Site U1369; IODP Site U1370; IODP Site U1371; laboratory studies; marine sediments; methods; microorganisms; Pacific Ocean; sediments; South Pacific;

SEDIS - Publications

Morono, Yuki et al. (2011): Preliminary experiment for cell count using flow cytometry