The onset of sustained Antarctic glaciation across the Eocene-Oligocene transition (EOT) marks a pivotal change in Earth's climate, but our understanding of this event, particularly the role of the carbon cycle, is limited. To help address this gap we present the following paleoceanographic proxy records from Ocean Drilling Program Site 1218 in the eastern equatorial Pacific (EEP): (1) stable isotope (delta (super 18) O and delta (super 13) C) records generated in epifaunal benthic foraminifera (Cibicidoides spp.) to improve (double the resolution) the previously published records; (2) delta (super 18) O and delta (super 13) C records measured on Oridorsalis umbonatus, a shallow infaunal species; and (3) a record of benthic foraminifera accumulation rate (BFAR). Our new isotope data sets confirm the existence at Site 1218 of a two-step delta (super 18) O increase. They also lend support to the hypothesized existence of a late Eocene transient delta (super 18) O increase and early Oligocene Oi-1a and Oi-1b glacial maxima. Our record of BFAR indicates a transient ( approximately 500 kyr) twofold to threefold peak relative to baseline Oligocene values associated with the onset of Antarctic glaciation that we attribute to enhanced biological export production in the EEP. This takes the same general form as the history of opal accumulation in the Southern Ocean, suggesting strong high-to-low-latitude oceanic coupling. These findings appear to lend support to the idea that the EOT delta (super 13) C excursion is traceable to increased organic carbon (C (sub org) ) burial. Paradoxically, early Oligocene sediments in the EEP are extremely C (sub org) -poor, and proxy records of atmospheric pCO (sub 2) indicate a transient increase associated with the EOT.