Morono, Yuki; Kallmeyer, Jens; Inagaki, Fumio; D'Hondt, Steven; Alvarez Zarikian, Carlos; Evans, Helen; Dubois, Nathalie; Engelhardt, Tim; Ferdelman, Timothy; Gribsholt, Britta; Harris, Robert N.; Hoppie, Bryce W.; Hyun, Jung-Ho; Kim, Jinwook; Lynch, Jill E.; Mitsunobu, Satoshi; Murray, Richard W.; Reese, Brandi K.; Shimono, Takaya; Shiraishi, Fumito; Smith, David C.; Smith-Duque, Christopher E.; Spivack, Arthur J.; Steinsbu, Bjorn Olav; Suzuki, Yohey; Szpak, Michal; Toffin, Laurent; Uramoto, Goichiro; Yamaguchi, Yasuhiko; Zhang Guoliang; Zhang Xiaohua; Ziebis, Wiebke (2011): Preliminary experiment for cell count using flow cytometry. IODP Management International, Washington, DC, United States, In: D'Hondt, Steven, Inagaki, Fumio, Alvarez Zarikian, Carlos, Evans, Helen, Dubois, Nathalie, Engelhardt, Tim, Ferdelman, Timothy, Gribsholt, Britta, Harris, Robert N., Hoppie, Bryce W., Hyun, Jung-Ho, Kallmeyer, Jens, Kim, Jinwook, Lynch, Jill E., Mitsunobu, Satoshi, Morono, Yuki, Murray, Richard W., Reese, Brandi K., Shimono, Takaya, Shiraishi, Fumito, Smith, David C., Smith-Duque, Christopher E., Spivack, Arthur J., Steinsbu, Bjorn Olav, Suzuki, Yohey, Szpak, Michal, Toffin, Laurent, Uramoto, Goichiro, Yamaguchi, Yasuhiko, Zhang Guoliang, Zhang Xiaohua, Ziebis, Wiebke, Proceedings of the Integrated Ocean Drilling Program; South Pacific gyre subseafloor; Expedition 329 of the riserless drilling platform; Papeete, Tahiti, to Auckland, New Zealand Sites U1365-U1371, 9 October-13 December 2010, 332, georefid:2012-014432

Abstract:
One of the major challenges in microbial ecology is to evaluate the accurate number of living cells in a natural environment. Cell count using flow cytometry is a powerful, high-throughput technique that has widely been used for aquatic habitats but not for sedimentary environments because mineral grains interfere with cell detection. During Integrated Ocean Drilling Program Expedition 329, we tested several sample preparation methods for onboard cell counting using flow cytometry with various pelagic sediments from the South Pacific Gyre. The cell numbers acquired from shallow sediments are almost consistent with microscopic direct counts (i.e., approximately 105 cells/cm (super 3) ). Yet, the method needs improvement and standardization to reduce the background signal and to lower the minimum detection limit for deep sedimentary habitats with very low cell densities (i.e., <1000 cells/cm (super 3) ).
Coverage:
West: -165.3900 East: -123.0900 North: -23.5100 South: -45.5800
Relations:
Expedition: 329
Site: 329-U1365
Site: 329-U1366
Site: 329-U1367
Site: 329-U1368
Site: 329-U1369
Site: 329-U1370
Site: 329-U1371
Data access:
Provider: SEDIS Publication Catalogue
Data set link: http://sedis.iodp.org/pub-catalogue/index.php?id=10.2204/iodp.proc.329.110.2011 (c.f. for more detailed metadata)
Data download: application/pdf
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